By Walker J.M. (ed.), Pollard J.W. (ed.)

CIP cites the sequence because the major access. Covers equipment for developing mammalian fibroblastic mobile cultures and retaining tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. recognition is given to the range of tradition media and extracellular matrices had to retain the differential services of the classy cells. additionally describes tradition concepts for either residing and glued cells.

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And Achong, B. ), William Heinemann Medical Books, London, p. 249. Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. , Aalesed, H. ), IARC, Lyon, France, p. 129. Miller, G. and Lipman, M. (1973) Release of infectious Epstein-Barr virus by transformed marmoset leucocytes. Nutl. Acud. USA 70,190. Chapter 6 Scale-Up of Suspension and Anchorage-Dependent Animal Cells J, Bryan Griffiths 1. Introduction In this chapter, scale-up is described in a laboratory context (lo-20 L), but the principles and techniques employed have been successfully adapted so that cells are now grown industrially in unit volumes of up to 8000 L for vaccine, interferon, and monoclonal antibody production.

Crawford, D. H. (1986) Use of the virus to prepare human-derived monoclonal antibodies, in The Epstein-BarrVirus: RecentAdz~ances (Epstein, M. A. and Achong, B. ), William Heinemann Medical Books, London, p. 249. Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. , Aalesed, H. ), IARC, Lyon, France, p. 129. Miller, G. and Lipman, M. (1973) Release of infectious Epstein-Barr virus by transformed marmoset leucocytes. Nutl. Acud. USA 70,190. Chapter 6 Scale-Up of Suspension and Anchorage-Dependent Animal Cells J, Bryan Griffiths 1.

For those cells that grow individually in suspension, the range of fermentation equipment developed for bacteria can be readily modified. This is a great advantage, since these culture vessels are economic in terms of space, the environment is homogeneous and can be critically controlled, and scale-up is relatively straightforward. Many cell types, however, will only grow when attached to a substrate or, in some cases, will only produce significant levels of a product when grown in this mode. Scale-up of substrate attached cells is far more difficult to achieve and has given rise to a wide range of alternative culture systems.

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