By Dr Desmond S. T. Nicholl

Des Nicholl offers a brand new, absolutely revised, and elevated variation of his well known undergraduate-level textbook. The publication keeps a number of the beneficial properties of the unique version and nonetheless deals a concise technical creation to the topic of genetic engineering. it really is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and sleek functions of genetic engineering. functions coated within the booklet comprise genomics, protein engineering, gene treatment, cloning, transgenic animals and vegetation, and bioethics. An creation to Genetic Engineering is vital examining for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.

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Additional resources for An Introduction to Genetic Engineering (Studies in Biology)

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Perhaps the best known example of this arrangement is the lac operon (Fig. 6), which codes for the enzymes responsible for lactose catabolism. Within the operon there are three genes that code for proteins (termed structural genes) and an upstream control region encompassing the promoter and a regulatory site called the operator. In this control region there is also a site which binds a complex of cyclic AMP and CRP (cyclic AMP receptor protein), which is important in positive regulation (stimulation) of transcription.

Fig. 7. Structure and expression of the mammalian ␤-globin gene. The gene contains two intervening sequences or introns. The expressed sequences (exons) are shaded and numbered. The primary transcript is processed by capping, polyadenylation and splicing to yield the fully functional mRNA. 6% of this length of DNA. The presence of introns obviously has important implications for the expression of genetic information in eukaryotes, in that the introns must be removed before the mRNA can be translated.

The data can be used to estimate the sizes of unknown fragments by construction of a calibration curve using standards of known size, as migration is inversely proportional to the log10 of the number of base-pairs. 3). In addition to its use in the analysis of nucleic acids, polyacrylamide gel electrophoresis (PAGE) is used extensively for the analysis of proteins. The methodology is different from that used for nucleic acids, but the basic principles are similar. One common technique is SDS–PAGE, in which the detergent SDS (sodium dodecyl sulphate) is used to denature multisubunit proteins and cover the protein molecules with negative charges.

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